FastQCFastQC Report
Fri 29 Apr 2016
ERR1042776.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042776.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11343991
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT545260.480659760749105No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT536090.4725761859296257No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT342980.30234509177590146No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT254210.22409220881786665No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA203000.017.1330051
TTAACGG22250.013.63595535
TAACGGC22600.013.3429236
GTATTAG39150.012.5696041
GTATCAA286300.012.1287112
ATTAGAG32100.011.8146423
AACGGCC25950.011.76300637
CTCTAAT33550.011.7451561
TAATACT43050.011.3019754
CTAATAC43000.011.1430233
TCTAATA39100.011.1189252
AACGCAG327000.011.0943425
AATACTG44750.011.0379895
GGGTAAG16400.010.9420731
TATTAGA37950.010.9196312
ACCGCCT30950.010.81906212
TTTAACG28750.010.81043434
TAGGACC21400.010.8060744
CGAACTA30650.010.80424124
AGGTCGC25650.010.60233935
Produced by FastQC (version 0.11.5)