FastQCFastQC Report
Fri 29 Apr 2016
ERR1042774.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042774.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14058715
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1277770.9088810748350755No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1132300.8054078911194942No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT671860.47789573940434815No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT559290.39782440998341595No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT198310.14105841109944972No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT152910.10876527477795801No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGAAT18650.018.35120632
GCGAATC20250.016.35308533
GACTAAG24650.015.53549726
TAAGGCG24550.015.22199629
ACGAGAC26650.014.43902522
AATCTCG19400.014.30412436
GGTATCA541800.014.170361
CCCACGA27250.014.12110119
TCTCCGA27350.014.06946911
ATCTCCG27650.014.05063310
CGAGACT28000.013.808928523
AGACTAA28100.013.69395125
AGGCGAA26650.013.60600431
CACGAGA28250.013.42477921
ATCTCGT17350.013.32853137
CGAATCT25500.013.13137234
ACTAAGG29800.012.91275127
CGAGCCC29700.012.89393915
GCCCACG31950.012.73865318
TATACAC39000.012.712823
Produced by FastQC (version 0.11.5)