FastQCFastQC Report
Fri 29 Apr 2016
ERR1042773.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042773.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6846461
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1074001.568693665238143No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT696141.0167880894961645No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT510870.7461811291994507No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT412630.602690937697593No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT175410.2562053592359615No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT133650.19521034297865716No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA107360.15681094217874023No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106270.15521887877547247No Hit
GAGTACGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA70580.10308975688315468No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGT10700.020.74766337
CCCACGA13200.018.07954619
ACGAGAC13700.017.82481822
ATCTCCG14400.016.8298610
TCTCCGA14400.016.7013911
GCCCACG14850.016.19528618
CACGAGA15600.015.53525521
CGAGCCC15850.015.2902215
TATACAC20900.015.0478473
TACACAT19950.015.0225565
GATCTCG16800.014.09523836
AACGCAG544450.012.8339625
GTATTAG20750.012.4819281
GGATCTC19800.012.42676835
TATTAGA18700.012.366312
CCGAGCC21700.012.36175214
ATACACA25750.012.1417474
ATTAGAG17100.012.116963
TTAACGG13000.012.09615435
ACACATC22450.012.031186
Produced by FastQC (version 0.11.5)