FastQCFastQC Report
Fri 29 Apr 2016
ERR1042772.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042772.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7675783
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT677240.8823073815400981No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT603330.7860175307196673No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT360500.4696589260014255No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT309730.4035158367556769No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117180.15266194992745366No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT87600.11412516482031865No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA244850.016.1464161
TAACGGC15400.014.77597336
TTAACGG15400.014.77597335
GTATTAG28500.014.2807021
CTAGTAC4350.013.1839083
TATTAGA23950.012.8997922
TAATACT26900.012.7918224
ACCGCCT19300.012.55699512
ATTAGAG22550.012.5521073
GGTCTAG5650.012.4424771
AACGGCC18650.012.30026937
AACGCAG378450.012.1377995
GTATCAA336100.011.75721552
TTTAACG20600.011.58495234
AATACTG27650.011.5750465
TATGCGG9300.011.3387115
TCTATAC8200.011.2804873
GGTAAAC18250.011.15068535
CGAACTA20250.011.145678524
TCTAATA23750.011.13894752
Produced by FastQC (version 0.11.5)