FastQCFastQC Report
Fri 29 Apr 2016
ERR1042771.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042771.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6702230
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1142371.7044625445560655No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT735751.0977689515280735No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT568040.8475388042487351No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT412930.6161083699007643No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT182520.2723272701772395No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT143910.2147195784089773No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120560.17988042785759367No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA115320.17206213454327887No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGT94200.14055023477260553No Hit
GAGTACGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA83190.12412286656829144No Hit
GTACGGGAAGCAGTGGTATCAACAAAAAAAAAAAAAAAAAAAA72610.10833707586877801No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71000.10593489032754769No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGT16350.021.27217337
ACGAGAC18400.020.5108722
ATCTCCG19200.019.46354310
TCTCCGA19600.019.3494911
CCCACGA19550.019.0204619
CGAGACT20250.018.54567723
CGAGCCC20500.018.31951315
ACTAGGC20300.018.31773427
AGACTAG20650.017.91767725
CACGAGA21000.017.88333321
GACTAGG20950.017.74940526
GCCCACG21200.017.6273618
GAGACTA21200.017.6273624
TATACAC23800.017.5672283
TAGGCAT21700.017.4769629
CTAGGCA21900.017.14840128
GATCTCG21350.016.89695536
ACATCTC26300.015.4752858
GCATGAT25250.015.23960432
ATACACA29800.015.2097314
Produced by FastQC (version 0.11.5)