FastQCFastQC Report
Fri 29 Apr 2016
ERR1042770.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042770.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8603446
Sequences flagged as poor quality0
Sequence length43
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT2072142.408500035915841No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1187751.3805514674003883No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1048341.2185117451774556No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT678670.7888350784092792No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT335270.38969268825538045No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT265690.30881811776351015No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA247250.28738484556072064No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT224510.26095357604383174No Hit
GAGTACGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA156270.1816365209940296No Hit
GTACGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA135970.15804132437165294No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT132640.15417078226561776No Hit
GTACGGGAAGCAGTGGTATCAACAAAAAAAAAAAAAAAAAAAA132140.1535896197872341No Hit
GAGTACGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA124020.14415154113828343No Hit
GAGTACGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAA113870.1323539428270951No Hit
GAGTACGGGAAGCAGTGGTATCAACAAAAAAAAAAAAAAAAAA112040.1302268881562109No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGT110390.1283090519775448No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA109550.12733269901386027No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109420.12718159676948051No Hit
AAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA106200.12343891040868973No Hit
GTACGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAAAA101070.11747618338047337No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93740.10895634144736889No Hit
CAGTTGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA93090.10820083022547013No Hit
GTACGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAA92940.10802648148195503No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGT20350.023.45454637
GACGGAC20850.023.06954426
CGAGACG20950.022.87112223
TATCTCG21700.021.9101436
CCCACGA22600.021.6106219
ACGAGAC22850.021.37417822
ACGGACT23150.020.8574527
GCCCACG24450.020.27811818
CGAGCCC24200.020.10537115
TCTCCGA24350.020.05749511
AGACGGA24250.019.91133925
CGGACTC25200.019.30753928
ATCTCCG26250.018.60571510
CACGAGA26300.018.35931621
CCTATCT27050.018.12384434
GAGACGG27300.017.95787624
CTATCTC28900.016.96366935
TCCTATC29700.016.31986433
TACACAT35350.016.118815
AGCCCAC31200.016.0689117
Produced by FastQC (version 0.11.5)