FastQCFastQC Report
Fri 29 Apr 2016
ERR1042769.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042769.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9175078
Sequences flagged as poor quality0
Sequence length43
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1736931.893095622729311No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1138981.2413845419079816No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT878960.9579864062191079No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT685940.7476121728883395No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT305280.3327274165952595No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT237400.25874439432558505No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT190310.20742057996673163No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA188040.20494648655847938No Hit
GTACGGGAAGCAGTGGTATCAACAAAAAAAAAAAAAAAAAAAA124400.13558467840818356No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118940.1296337753204932No Hit
GTACGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA106470.11604261020996226No Hit
GAGTACGGGAAGCAGTGGTATCAACAAAAAAAAAAAAAAAAAA100670.10972113806552924No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99880.10886010996309786No Hit
GAGTACGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA97320.106069942947624No Hit
GAGTACGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA96760.10545959391298908No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCACGA9650.016.29533819
ATCTCGT8750.016.0685737
ACGAGAC10300.014.90776722
TTAACGG17100.013.956140535
CGAGCCC11650.013.81545215
TAACGGC17400.013.60919636
ATCTCCG12100.013.1487610
TCTCCGA11400.013.14473611
TATTAGA28600.012.7430082
GTATTAG34550.012.4225761
AACGCAG916300.012.3501595
ACCGCCT20950.012.27446312
TAATACT31400.012.0191094
ATTAGAG25200.011.96626953
GTCGGGA9000.011.9222222
CGAGACT13650.011.65567823
GGCACCG21950.011.4624149
CATCTCG12600.011.4523836
TTATGCG10350.011.2608694
AACGGCC20900.011.24162737
Produced by FastQC (version 0.11.5)