FastQCFastQC Report
Fri 29 Apr 2016
ERR1042768.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042768.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7508536
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1094741.4579939418283405No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT749840.9986500697339667No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT504700.672168316167093No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT416170.5542625086967685No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT163960.2183648050698565No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT132660.17667891583658918No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA130490.17378887175875563No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97970.13047816511767407No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC94640.1260432126848696No Hit
GTACGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA88020.1172265805211562No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG78510.10456099564548935No Hit
GAGTACGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA78430.10445445024169825No Hit
GAGTACGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA77850.1036819960642128No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG76120.10137795170723027No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGT9100.015.85714237
GGGTAAG10250.015.1609751
TTAACGG24300.014.69341735
CGAGACA10250.014.25853623
GTATTAG36750.014.1959191
TAACGGC25050.014.17964136
ATTAGAG30050.014.0366053
ACGAGAC11100.013.83333322
CCGAGTT9950.013.57286513
GTGACAC27750.013.53333424
ACCGCCT28400.013.48415612
TATTAGA31150.013.4221522
GGCACCG28450.013.3954319
AACGGCC27000.013.22407437
GCCGAGT9800.013.21428512
CCCACGA11100.013.16666719
GTAAGAT12200.012.8893443
AATCTCG11500.012.86956536
GACACAT29950.012.8480826
CACATGT29450.012.8149428
Produced by FastQC (version 0.11.5)