FastQCFastQC Report
Fri 29 Apr 2016
ERR1042767.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042767.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8919767
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT949771.064792387514158No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT667980.7488760636909013No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT470580.5275698345035246No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT371080.4160198354957029No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT164880.1848478777528606No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT119250.13369183298173595No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97910.10976744123473181No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA97640.10946474274496183No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAACGG15400.015.256494535
TAACGGC16250.014.23076936
TAATACT31500.013.6841274
GTATTAG30300.013.1270631
AACGGCC18150.012.84297537
CTCTAAT23150.012.7861781
GACCGTA4700.012.59574526
AATACTG33600.012.4434525
GGGTAAG11200.012.3883931
AACGCAG507450.012.3588535
TTTAACG19850.012.0226734
TACCCCG11350.011.8986795
CTAATAC31850.011.675043
TCTAATA27950.011.4508062
TACTGGT27100.011.2638377
ATGCTAG23750.011.21684315
TATTAGA27750.011.22
TATACCG3650.011.1506855
ATTAGAG24250.011.1381443
GATGCTA24950.010.97394814
Produced by FastQC (version 0.11.5)