FastQCFastQC Report
Fri 29 Apr 2016
ERR1042765.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042765.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5771646
Sequences flagged as poor quality0
Sequence length43
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1810843.1374758604391193No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT916511.587952552876597No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT877571.5204847975776754No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT534940.9268413204829264No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT240240.4162417445560591No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA216520.3751442829307272No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT213310.3695826112689517No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT176150.30519889819992424No Hit
CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGT169380.2934691420783603No Hit
GTACGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA118210.20481159100887336No Hit
GAGTACGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA111270.19278729152827462No Hit
GTACGGGAAGCAGTGGTATCAACAAAAAAAAAAAAAAAAAAAA110810.1919902918508862No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108330.1876934240249662No Hit
GAGTACGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA106620.18473066435467456No Hit
GAGTACGGGAAGCAGTGGTATCAACAAAAAAAAAAAAAAAAAA94220.16324632522507443No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86140.14924685263094792No Hit
AAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA82280.14255898577286272No Hit
GAGTACGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAA78270.1356112277156291No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75420.13067329493180976No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA69770.12088405976388712No Hit
GTACGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAAAA67010.11610206169955677No Hit
CAGTTGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA66620.11542634458177095No Hit
GAGTACGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA62800.10880778204345866No Hit
GTACGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAA61380.10634747869152057No Hit
TATCAACGCAGAGTACGGGAACAGTGGTATCAACGCAAAAAAA60290.10445893597770894No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCACGA30150.024.23714819
ATCTCGT27850.024.0466837
ACGAGAC30600.023.51797322
ATCTCCG31200.023.42147410
TCTCCGA31550.023.04437411
CACGAGA31800.022.80503321
GCCCACG32300.022.68111418
CGAGCCC32850.022.18873815
TATACAC35000.021.8299983
GATCTCG32800.021.43292836
GGATCTC33150.021.1508335
TCCGAGC35800.020.5670413
TACACAT37900.020.501325
CGAGACC35250.020.36312123
CCGAGCC37000.020.314
ACACATC37600.020.1236696
ACATCTC37650.019.8512638
AGCCCAC38050.019.44809517
GACCAGA37000.019.426
CCACGAG38400.019.0299520
Produced by FastQC (version 0.11.5)