FastQCFastQC Report
Fri 29 Apr 2016
ERR1042763.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042763.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4606487
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1099192.3861784479148644No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT623441.353395765580148No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT537441.1667025219000944No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT347960.7553695473361804No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT177900.38619451221722756No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT143200.31086595924399657No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA116070.2519707534179517No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104920.22776575728966567No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71920.15612765215662175No Hit
GAGTACGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA69230.1502880611624433No Hit
GTACGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA64750.14056264567771493No Hit
GTACGGGAAGCAGTGGTATCAACAAAAAAAAAAAAAAAAAAAA58550.12710336531938546No Hit
GAGTACGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA56700.12308728972859362No Hit
AAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA52130.11316649759350239No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49640.10776107693346361No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48790.10591585301337006No Hit
GAGTACGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAA48590.1054816826792304No Hit
GAGTACGGGAAGCAGTGGTATCAACAAAAAAAAAAAAAAAAAA48200.10463505052765806No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA46320.10055384938674526No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGT7350.020.38775437
CCCACGA8950.018.39664619
AGACTAG9150.017.99453525
GCCCACG9500.017.72105218
CGAGCCC9450.017.22751415
TATACTG3450.017.159425
TCTCCGA10100.017.03465511
ATCTCCG10050.016.93532210
ACGAGAC9850.016.71573622
GAGACTA10350.016.08695624
GACTAGG10450.015.57894726
CGAGACT10850.015.5161323
TAGGCAT11100.015.33333329
CACGAGA11150.015.26457421
GATCTCG10650.015.11267636
ACTAGGC11200.015.03124927
CTAGGCA11800.014.26694928
TATACAC14100.014.0390063
TTACTCG2900.014.03448319
GTCGGGA5900.013.796612
Produced by FastQC (version 0.11.5)