FastQCFastQC Report
Fri 29 Apr 2016
ERR1042762.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042762.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4619999
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT649671.4062124255871051No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT367480.795411427578231No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT289330.6262555468085599No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT208620.45155853929838513No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95220.2061039407151387No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT79780.1726840200614762No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA63410.13725111195911516No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54580.11813855370964366No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGACG5300.016.75471723
TATCTCG5400.016.1018536
ATCTCGT5600.014.86607137
GACGGAC6200.014.62096726
GTATTAG16000.014.1062511
TTAACGG8950.013.84916235
CCCACGA6550.013.83969419
ACGAGAC6700.013.8059722
AACGCAG291950.013.67460255
TAACGGC9250.013.636
ATCTCCG7350.013.59183610
TATTAGA13750.013.1854542
ACGGACT7150.012.93706227
ATTAGAG12950.012.7142853
TATATCG1807.476592E-712.3333345
TTTAACG10800.012.33333334
CCGAGTT4650.012.33333313
AACGGCC10700.012.27570137
CGGACTC7850.012.25477728
TTAATAC6150.012.032523
Produced by FastQC (version 0.11.5)