FastQCFastQC Report
Fri 29 Apr 2016
ERR1042760.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042760.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6485011
Sequences flagged as poor quality0
Sequence length43
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1536412.369171000635157No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT755711.165317992521524No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT710891.0962047712794938No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT440960.6799680062223488No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT236690.36498010566211836No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT190360.2935384380997966No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA177990.2744636824825741No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGT157240.2424668207964489No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT143140.2207243750241904No Hit
GAGTACGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA106140.163669730089895No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96770.14922102676464233No Hit
GTACGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA93930.1448416972615775No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA83350.12852715284523034No Hit
GAGTACGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA76580.11808769483968493No Hit
AAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA76480.11793349309661925No Hit
GTACGGGAAGCAGTGGTATCAACAAAAAAAAAAAAAAAAAAAA72630.11199672598859121No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72100.11117945675034321No Hit
GAGTACGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAA68480.1055973536513662No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67250.10370067221165856No Hit
CAGTTGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA66360.10232827669837415No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGT26450.023.99054737
CGAGACA28650.023.31064623
CCCACGA29600.022.87519
ACGAGAC29600.022.687522
AATCTCG28550.022.6795136
ATCTCCG30200.022.4205310
CACGAGA30500.022.26065621
TCTCCGA30500.022.13934511
CGAGCCC31050.021.80676515
TACACAT34800.021.1048855
GCCCACG32000.020.98593918
TATACAC35350.020.200853
ACACATC36600.019.3592916
TCCGAGC35650.019.14866813
CCGAGCC36950.019.07577914
GACAGGC36100.018.75623326
ATACACA40000.018.6850014
CCACGAG36700.018.44959320
AAATCTC35800.018.18994535
ACATCTC40400.018.042088
Produced by FastQC (version 0.11.5)