FastQCFastQC Report
Fri 29 Apr 2016
ERR1042759.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042759.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3874658
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT440241.136203504928693No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT272170.7024361892068925No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT213930.5521261489401129No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT119410.3081820382598929No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79310.20468903319983336No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT59930.15467171554237819No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGTA5150.021.19417426
ACCGTAC5400.019.8703727
CGTACTA5650.019.31858429
GTACTAG6550.016.66412230
CTAGATC7400.016.033
ACTAGAT7700.015.37662332
ATCTCGT7300.015.20548137
ATCTCCG7800.015.17948710
TCTCCGA7600.014.84868411
AGACCGT7250.014.825
CACGAGA7700.014.65584421
CGAGCCC7900.014.51898715
TACTAGA7700.014.41558431
ACGAGAC7900.014.2848122
CCCACGA8700.013.82183819
GAGACCG7950.013.72955924
TATACAC11700.013.598293
AACGCAG207150.012.8334545
TCCGAGC9300.012.73118413
TATACTG7400.012.255
Produced by FastQC (version 0.11.5)