FastQCFastQC Report
Fri 29 Apr 2016
ERR1042758.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042758.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5760007
Sequences flagged as poor quality0
Sequence length43
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1636362.840899325295959No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT848101.4723940439655716No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT715321.2418734907787439No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT481440.8358323175648918No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT210360.36520788950430094No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT186120.3231246073138453No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA165060.28656215174738503No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT135440.23513860313016982No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGT111220.19309004311973926No Hit
GAGTACGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA106530.18494769190384666No Hit
GTACGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA93400.1621525807173498No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90980.15795119693430928No Hit
GTACGGGAAGCAGTGGTATCAACAAAAAAAAAAAAAAAAAAAA89320.15506925599222363No Hit
GAGTACGGGAAGCAGTGGTATCAACAAAAAAAAAAAAAAAAAA83240.14451371326458456No Hit
GAGTACGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA82230.14276024317331557No Hit
GAGTACGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAA75190.13053803580447038No Hit
AAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA72930.12661442946163087No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69040.11986096544674338No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA63240.10979153323945615No Hit
CAGTTGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA61430.106649175947182No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61360.10652764831709405No Hit
GAGTACGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA60700.10538181637626481No Hit
GTACGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAAAA60660.10531237201621456No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAATC20350.024.45454633
GGCGAAT20800.024.10336532
ACGAGAC22450.023.15590322
GACTAAG22700.023.06387726
ATCTCGT19000.022.88157837
CACGAGA22650.022.86975721
CGAATCT21650.022.81524334
CCCACGA22750.022.60659219
TAAGGCG23300.022.23175829
AATCTCG20950.022.16467736
ACTAAGG24150.021.98550627
TCTCCGA23650.021.98097211
CGAGACT23900.021.90585723
CGAGCCC23700.021.85653915
AGACTAA23800.021.84243625
ATCTCCG24350.021.50102810
TATACAC28100.021.001783
AGGCGAA24600.020.98170731
CTAAGGC25050.020.75249528
GCCCACG25000.020.64618
Produced by FastQC (version 0.11.5)