FastQCFastQC Report
Fri 29 Apr 2016
ERR1042757.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042757.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3035546
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT726132.3920902532855703No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT355911.1724744082283716No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT313211.0318077868034286No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110580.364283723587124No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT95990.31621988268337886No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT88860.2927315217756542No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA52740.1737413961112762No Hit
GAGTACGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA47450.15631454769586756No Hit
GAGTACGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA43110.14201728453464385No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42640.1404689634088892No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42190.13898652828848582No Hit
AAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA40470.13332033182827735No Hit
GAGTACGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAA37520.1236021460389663No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG31600.10409988845499293No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACAC9550.016.2722513
GGGTAAG3800.016.065791
CGAACTA6450.016.06201624
ACGAGAC6750.015.89629622
AGGTCGC5500.015.80909235
GCTCGGA6300.015.56349211
ATCTCGT6500.015.08461537
GCGAACT7000.015.06428523
GTCGCCC6300.014.9761937
CCCACGA7200.014.90277919
GCTCCGA6350.014.85826829
ATCTCCG7550.014.45695410
AGCGAAC7450.014.40268422
TGCTCGG7350.014.34693910
GCCCACG7750.014.32257918
AACGCAG331800.014.2848095
GGTCGCC6100.014.25409936
TCTCCGA7350.014.09523911
GTACGAC1203.3038676E-513.8749993
TACACAT10500.013.7428585
Produced by FastQC (version 0.11.5)