FastQCFastQC Report
Fri 29 Apr 2016
ERR1042756.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042756.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7298935
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT738551.0118599494309786No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT487540.6679604627250414No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT350060.4796042162315461No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT279170.38248045776541373No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120030.16444864901523304No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT92400.1265938112889072No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG14150.013.20494651
AACGCAG356000.012.8304785
TTAACGG6750.012.33333435
TAATACT16850.012.2967374
GTACTAG4300.012.0465121
TACACTG18800.011.1196815
TAACGGC7350.011.07482936
GGTATCA310750.011.0315361
TATTAGA15000.010.9766662
ACGCAGA420700.010.8792496
GAGTACG225750.010.874641
CGCAGAG420000.010.8709537
AGTACGG224400.010.8658652
ATCTCGT7550.010.78145737
TCTCCGA9000.010.68888911
CTAGACT7050.010.4964544
AGAGTAC437450.010.36541410
CAGAGTA443150.010.29888259
GCAGAGT445000.010.251918
TAAGGCT9250.010.24
Produced by FastQC (version 0.11.5)