FastQCFastQC Report
Fri 29 Apr 2016
ERR1042755.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042755.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4882088
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1149912.3553651634300734No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT565731.1587869780307114No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT500861.025913502583321No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT263080.5388677959102744No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT168530.34520066004545596No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT136180.2789380281551664No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA115000.23555495107830915No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84950.17400341820958573No Hit
GAGTACGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA78800.16140634908670223No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67720.13871114162628775No Hit
AAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA63120.1292889435831554No Hit
GAGTACGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA60050.12300065054132575No Hit
GTACGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA59710.12230422720770293No Hit
GAGTACGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAA55830.11435680798871303No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGT52180.10688049867187974No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGT10800.019.52777937
TCTCCGA12100.018.511
ATCTCCG12550.018.13147410
CGAGCCC12800.017.77734415
TATACAC14550.017.5463923
ACGAGAC12650.017.11067222
CCCACGA12700.017.04330619
AGACTAG13250.016.3358525
GAGACTA13900.015.83812924
GCCCACG14150.015.81978918
TACACAT15900.015.7075465
CGAGACT14500.015.31034523
ACTAGGC14700.015.22789227
ATACACA18150.015.1873274
TTTAGCG1601.0990334E-815.0312526
GACTAGG14600.014.95205526
AGGTCGC9000.014.59444435
CTAGGCA15300.014.50980428
CACGAGA14950.014.4782621
TAGGCAT15350.014.34201929
Produced by FastQC (version 0.11.5)