FastQCFastQC Report
Fri 29 Apr 2016
ERR1042754.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042754.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6354285
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT650861.0242851870824177No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT400990.6310544774116993No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT286380.45068800030215833No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT225870.3554609212523518No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105010.16525856174219444No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT80260.126308467435754No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGGC11600.016.58620636
TTAACGG12050.015.81327835
AACGGCC13700.013.90875937
GTATTAG23050.013.8850331
TTTAACG14450.013.31487934
ATTAGAG17750.013.1323943
TATTAGA19500.012.9974372
AACGCAG306000.012.8230395
TACCCCG8350.012.6287425
ACCGCCT16950.012.005912
CGAGACG5400.011.99074123
TAATACT20750.011.9469884
GGGTAAG6950.011.712231
GGCACCG17550.011.7008549
TGCGTCA10000.011.65500110
GTTTAAC17450.011.55587433
CTTACCC10600.011.3443393
GACGGAC5400.011.30555626
CTAGCAC8750.011.2057143
TATCTCG5550.011.036
Produced by FastQC (version 0.11.5)