FastQCFastQC Report
Fri 29 Apr 2016
ERR1042753.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042753.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7975648
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1155931.449324242995679No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT588100.7373695529190858No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT582790.7307117866786498No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT328010.41126438879950566No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT189810.2379869322216828No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGT177910.22306651447004683No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT144750.1814899554243116No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT125110.15686499705102333No Hit
CAGTTGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA115840.14524211700416068No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTG102370.12835320716260296No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGT81690.10242427950681876No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGAGAC30500.022.07868822
CACGAGA30350.022.06589921
ATCTCGT30000.021.58333237
CCCACGA31700.021.18454219
CGAGACT32200.021.08540323
TCTCCGA32250.020.76589211
CGAGCCC33350.020.19190415
ATCTCCG34000.019.91470710
TATACAC36400.019.8722523
GCCCACG34100.019.69354818
CCACGAG34300.019.68658820
TCCGAGC35400.019.54519713
CCGAGCC36550.019.03146614
CATCTCG34750.018.95251836
TACACAT40200.018.73015
CTCCGAG37900.017.86543512
CTTATAC40900.017.0073341
ATACACA45300.016.9481264
GACTCCT39350.016.8780226
ACACATC41850.016.7538836
Produced by FastQC (version 0.11.5)