FastQCFastQC Report
Fri 29 Apr 2016
ERR1042752.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042752.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5848586
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT329990.5642218478107358No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT238350.4075344023324612No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT158740.27141603115693264No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT141090.24123779662297862No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGACA9500.017.3315823
ATCTCGT9700.016.2113437
CACGAGA10500.015.50476221
ACGAGAC10650.015.460093522
TTAACGG12000.014.95416635
GTATTAG17750.014.9042241
AATCTCG11150.014.434978536
TATACAC14950.014.2307693
TAACGGC12450.013.96787236
TCTCCGA11600.013.87511
GGGGTTA12400.013.7258076
ATCTCCG12150.013.70370410
CGAGCCC12250.013.59183715
GGGTTAG12650.013.3083017
GCCCACG12750.013.20392118
GGGTAAG7150.013.1958051
CGCGTAA1153.5810642E-412.86956510
CCCACGA12500.012.72819
TAATACT20150.012.6699754
ATTAGAG16400.012.6341463
Produced by FastQC (version 0.11.5)