FastQCFastQC Report
Fri 29 Apr 2016
ERR1042751.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042751.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6938521
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1081881.5592371919030008No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT526040.7581442788744173No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT523610.7546420915927183No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT285850.4119754051331689No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTG180200.2597095259926431No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGT147000.21186071210276655No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT142430.2052742940462384No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAG130420.18796512974450896No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT124770.17982218400722574No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCG119070.17160717680324092No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGT115480.16643316349406453No Hit
GAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTG115120.16591432093381286No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103810.1496140171659061No Hit
CAGTTGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA102280.14740893628483648No Hit
CCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTT100540.14490119724362008No Hit
TCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTG95270.1373059186532692No Hit
GGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAG93720.13507201318551892No Hit
CTTCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGT88030.12687141827487444No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTA82430.11880053400429286No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACAC29900.021.0367893
GACTAAG28100.020.73843426
GGCGAAT27450.020.55555532
GCGAATC27550.020.1451933
TAAGGCG28850.020.07105829
CACGAGA29700.019.62121221
AGACTAA29850.019.27470625
ACTAAGG30300.019.23267427
ATCTCCG30800.019.2207810
ACGAGAC30350.019.20098922
ATCTCGT24550.019.1405337
TACACAT33750.019.0207425
CCCACGA31000.018.91774419
TCTCCGA31200.018.79647411
AATCTCG27300.018.77106336
GAGACTA30850.018.70988724
CGAATCT29700.018.534
CGAGACT31950.018.23943723
AGGCGAA32400.018.0432131
CGAGCCC32350.018.0139115
Produced by FastQC (version 0.11.5)