FastQCFastQC Report
Fri 29 Apr 2016
ERR1042750.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042750.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9255283
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT895170.9671989500483129No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT573330.6194624194635647No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT419600.4533626902602546No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT355800.3844290876897011No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTG130460.1409573321528904No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124660.1346906410101128No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT104870.11330825864535962No Hit
CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGT102410.11065031722962983No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGT95600.10329235745681682No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAG94070.1016392475519117No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACAC25100.018.2788853
CCCACGA21900.017.48630119
TCTCCGA22250.017.46067211
ATCTCGT20350.017.45454637
ACGAGAC22850.017.0831522
ATCTCCG23750.016.35789510
CACGAGA24050.016.021
CGAGCCC24350.015.95482515
TTAACGG19850.015.09823735
TACACAT31850.014.6373635
TAACGGC20600.014.54854436
GCCCACG27500.014.39636318
GGGTAAG11200.014.3705351
GATCTCG25700.014.324903536
CGAGACC28350.014.02998323
ATACACA35600.013.7710694
ACACATC32000.013.7593766
TTATACA31050.013.584542
GTATTAG32150.013.4074651
ACATCTC33500.012.9223888
Produced by FastQC (version 0.11.5)