FastQCFastQC Report
Fri 29 Apr 2016
ERR1042748.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042748.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3754001
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTG180600.4810867125501565No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTG178580.47570578697235294No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGT171780.45759178007677676No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT167860.44714958786638576No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAG165320.4403834735259794No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT113970.30359608321894427No Hit
GGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAG104090.2772774967294894No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTA89970.2396642941757341No Hit
AAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAA84880.22610542724948662No Hit
TCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTG83890.2234682409514542No Hit
GGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGCAGAGT81280.21651565889300511No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78310.20860409999890783No Hit
ACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGA73120.19477885061831365No Hit
TATCAACGCAGAGTACGGAAGCAGTGGTATCAACGCAGAGTAC65970.17573250513252392No Hit
GTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAG62550.1666222251938665No Hit
CCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTT60320.1606818964619349No Hit
CAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGC59840.15940326068107066No Hit
ACGCAGAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAG53320.14203512465766527No Hit
GTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGCAGA50580.13473624540856544No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGT45070.12005857217406174No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC44790.11931270130189098No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCG44020.11726155640342131No Hit
CAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGCA42970.11446454063278086No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG40920.10900370031867333No Hit
AAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAAC40530.10796480874672118No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG39170.10434200736760592No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT37850.10082575897022936No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGACT1655.4569682E-1116.8181824
AGGTCGC10300.015.98543735
TATACAC6300.015.5634923
GGGTAAG5950.015.2352941
GCGCATA855.368175E-415.23529316
GGTCGCC11800.014.58050736
GTCGCCC11400.014.44298437
TGCTCGG13450.014.16728610
CCGAGGT12350.013.93117432
GTAAGAT5750.013.8347833
CGAACTA12200.013.34426124
TGTACTG16700.013.2934135
GCTCCGA13100.013.13358729
GCGAACT12800.013.007812523
CGTACGA1000.001833825512.95000112
CTGCTCG16200.012.9043219
ATGTACT16250.012.7507694
GGTAAAC9450.012.72486820
AGCGAAC13400.012.70149222
TTATACA7000.012.6857142
Produced by FastQC (version 0.11.5)