FastQCFastQC Report
Fri 29 Apr 2016
ERR1042747.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042747.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11458753
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1842601.608028377957008No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1023160.8929069332413396No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT920680.8034731178863879No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT565130.4931863004639336No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTG379840.331484586499072No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT288080.2514060648658715No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGT285560.24920687268501204No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAG256940.22423033291667951No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCG247840.21628880559690916No Hit
GAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTG217300.18963669083363607No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT215680.188222924431655No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT198860.1735441893197279No Hit
CCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTT198410.1731514764302887No Hit
CAGTTGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA185440.16183261826134135No Hit
TCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTG175210.15290494524142373No Hit
CTTCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGT173110.15107228509070753No Hit
GGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAG169040.1475204151795575No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTA139460.12170608791375467No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA138330.12071994221360736No Hit
GAGTACGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAA132690.1157979406659695No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123850.10808331412676406No Hit
AAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA121960.10643391999111945No Hit
GAGTACGGGAAGCAGTTGTATCAACGCAAAAAAAAAAAAAAAA120960.10556122468125459No Hit
GTACGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAAAA118120.10308277000123836No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGACG22650.020.25607123
GACGGAC22700.019.64096826
ATCTCGT23150.019.33909237
CACGAGA24400.019.10655821
TATCTCG23600.018.89194936
TATACAC28550.018.078813
ACGGACT25150.017.80119327
TCCTATC26150.017.54493333
ACTCCTA26050.017.4702531
CCCACGA27200.017.27573619
TCTCCGA26650.017.14634111
ACGAGAC27050.017.09796722
CGGACTC26450.016.92627528
CCTATCT27050.016.89279234
CTATCTC27500.016.81818235
ATCTCCG27650.016.66003610
TACACAT32600.016.4003075
CGAGCCC28700.016.17944315
TCCGAGC30750.015.28130113
GCCCACG30850.015.17179918
Produced by FastQC (version 0.11.5)