FastQCFastQC Report
Fri 29 Apr 2016
ERR1042746.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042746.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10904755
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT666380.6110912166298097No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT520770.4775623111202406No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT338880.3107635155489509No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT310850.28505913246102277No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTG113740.104303122812021No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAACGG26350.014.67362435
GTATTAG39650.014.6506941
TAACGGC27150.014.30939336
ATTAGAG32450.014.1956853
GGGTAAG14950.013.8595311
TAATACT38250.013.7843134
TATTAGA36750.013.4408152
AATACTG40150.013.36239055
ATCTCGT12750.012.76862737
TCTAATA37150.012.698522
ATGCTAG31900.012.642632515
CTCTAAT34850.012.6341471
ATAGGGG21100.012.5379143
CCGAGTT13650.012.33333213
TTTAACG33200.012.25903634
CGAGACA13750.012.24363723
GGTAAAC31350.012.156335
GGGGTTA21600.012.0763896
CTAATAC44050.012.0533493
TACTGGT37350.011.9866127
Produced by FastQC (version 0.11.5)