FastQCFastQC Report
Fri 29 Apr 2016
ERR1042745.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042745.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4527441
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT307280.6787056971035073No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT299180.660814795819537No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT145570.3215282098651313No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT90780.20051061957516397No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55300.12214405444488398No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC47870.10573301783501983No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGTCGC11350.015.9735735
GGTATCA137200.015.2099121
GTCGCCC12350.015.12955437
CGAACGT1853.0559022E-1015.04
GCTCCGA12450.014.56224929
CCGAGTT6100.014.55737713
GGTCGCC13250.014.24150936
GGGTAAG8150.014.0736191
CCGAGGT13150.013.64638732
TGCTCGG15050.013.029901510
GTATCAA161100.012.9419622
GTAGAAC5200.012.4519233
GCTCGGA15750.012.45079311
TATACTG6100.012.4344265
GGTAAGA9000.012.3333332
TCGAACG2255.7207217E-912.3333333
TCACGTA11800.012.22881325
GTAAGAT9850.012.2081223
CGAGTTC7450.012.16778514
GCCGAGT7150.012.16083812
Produced by FastQC (version 0.11.5)