FastQCFastQC Report
Fri 29 Apr 2016
ERR1042744.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042744.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8266699
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT846221.0236492220171558No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT639070.7730655247033913No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT369900.4474579272814941No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT355680.43025638165850716No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG162010.19597907217862898No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG157160.19011215964195624No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC155770.1884307146056727No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTG134220.16236226817983818No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128270.15516471568639428No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC115200.13935429365457724No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC114490.13849542604611587No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC112420.1359914035820102No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT101230.12245516620358379No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG100840.12198339385527403No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG99970.12093097861673686No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAG98280.11888663177406121No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGT96320.11651567330563264No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT95820.11591083696164575No Hit
ATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGT94910.11481003481558963No Hit
ACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGG91270.11040682623136513No Hit
AGGGATAACAGCGCAATCCTATTCTAGAGTCCATATCAACAAT89960.10882215501011952No Hit
ATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTC89420.1081689317586137No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTCGG1401.875378E-917.17857224
GCGAATC4750.016.7473733
CGCTATA803.3857356E-416.18752
CGAGTTC11800.015.83474514
CCGAGTT11950.015.3263613
CGAATCT5500.014.834
GCCGAGT12150.014.61728412
TGCGACG1956.7484507E-1014.2307722
AGGTCGC40500.014.06913635
CGTGCGA5400.013.70370410
GGTCGCC41750.013.64790436
CGCGATA950.001246215813.63157914
TTCGGTA1907.1468094E-913.63157926
GTCGCCC41700.013.61990437
GTATTAG42100.013.4026131
GGCACCG33250.013.2977459
GGGTAAG16150.013.2879261
TACCCCG19550.013.1534545
TATTAGA39450.013.1305452
GGCGAAT6350.013.11023532
Produced by FastQC (version 0.11.5)