FastQCFastQC Report
Fri 29 Apr 2016
ERR1042743.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042743.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6128911
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT547320.8930134570399212No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTG391350.6385310538854293No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT349970.5710149812911298No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT286380.467260823333868No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAG256530.41855722819274094No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCG243290.39695469554052915No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGT240380.39220670686847964No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT206780.3373845696241959No Hit
CCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTT190410.3106750938298827No Hit
GAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTG183890.3000369886265276No Hit
CTTCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGT147060.23994474711739167No Hit
GGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAG141460.2308077242433444No Hit
TCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTG136050.2219807075025237No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTA115980.18923427016642924No Hit
AAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAA108420.1768992892864654No Hit
ACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGA102050.16650592576723663No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92240.15049981962537878No Hit
GCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCACTG91910.14996138792030103No Hit
ACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATAC91180.14877031172421984No Hit
GTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAG89840.14658395267935853No Hit
CAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGC87710.14310862076476555No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGT81510.1329926311542132No Hit
GCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTG77740.12684145682650638No Hit
CCACTGCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTA72880.11891182626081534No Hit
GGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGCAGAGT69720.11375593478188865No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT67640.1103621834286711No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66440.10840424995566098No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACGTA6700.019.05223825
CGAGACG6800.019.04411723
ATCTCGT7650.017.16993537
CGTAGAG7550.016.66225228
AATCTCG8100.016.44444536
TATACAC11500.015.7652173
CTAGTAC3900.015.1794873
ACGTAGA8400.014.976191527
GACGTAG8500.014.826
ACGAGAC9400.014.36702222
CACGAGA9150.014.15300621
CCCACGA9850.013.89847719
GAGACGT9750.013.47179524
TCTCCGA9650.013.41968911
ATACACA15300.012.9379084
TATACTG7750.012.8903245
GAATCTC10450.012.74641235
ATCTCCG10600.012.74056510
TAATACT10350.012.6908214
TACACAT13700.012.5583945
Produced by FastQC (version 0.11.5)