FastQCFastQC Report
Fri 29 Apr 2016
ERR1042742.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042742.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6217290
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT809031.3012582652570492No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTG563740.906729459298183No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT470400.7565997404013646No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT423890.681792227803432No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAG385920.6207206033496909No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCG357230.5745750962235958No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGT337470.542792760189729No Hit
CCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTT280030.45040524086860995No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT270840.43562388114435713No Hit
GAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTG259290.4170466553755736No Hit
GGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAG215780.34706439622407836No Hit
CTTCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGT208660.3356124613778672No Hit
TCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTG197500.3176625185571205No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTA174250.28026680434723167No Hit
AAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAA158700.2552559073165318No Hit
ACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGA148490.2388339614204903No Hit
ACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATAC133390.214546852406756No Hit
GCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCACTG133330.21445034733782725No Hit
CAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGC131860.21208597314907301No Hit
GTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAG130520.20993069327633102No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT125760.20227462447465053No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGT124700.20056970159024268No Hit
GCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTG112010.18015887951181303No Hit
CCACTGCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTA107080.1722293796815011No Hit
GGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGCAGAGT100160.16109912839838578No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT96090.15455286788938588No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93020.1496150251958651No Hit
GTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCACTGCT83830.13483366547161224No Hit
GCGTTGATACCACTGCTTCCGTACTCTGCGTTGATACCACTGC79030.1271132599573126No Hit
TATCAACGCAGAGTACGGAAGCAGTGGTATCAACGCAGAGTAC78250.1258586940612389No Hit
CTGCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTC76370.12283486856813822No Hit
GCTTCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCG71770.11543614661693438No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70350.11315219331895407No Hit
GCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACG68660.11043396721079442No Hit
CAGTTGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA67230.10813392973465931No Hit
ACGCAGAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAG66760.10737797336138413No Hit
ATACCACTGCTTCCCGTACTCTGCGTTGATACCACTGCTTCCC65600.10551220869542839No Hit
GTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGCAGA65580.10548004033911881No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGT63460.10207019457030314No Hit
GATACCACTGCTTCCCGTACTCTGCGTTGATACCACTGCTTCC63230.10170025847274294No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGACA12850.017.85213923
ACGAGAC13650.017.34798622
AATCTCG12600.017.03174636
ATCTCGT12650.016.67193837
TATACAC14950.016.4581933
GTACTAG3600.016.4444451
CACGAGA13750.016.41454521
TAGACCG803.3855665E-416.18755
TTAACGG9550.016.07853335
CCCACGA14600.015.71232919
TCTCCGA14700.015.35374211
ATCTCCG15250.014.810
AGGCAAT15500.014.68064632
GGCAATC14900.014.65100833
TAACGGC10500.014.44761936
ATACACA18700.014.245994
TACACAT19050.014.1784775
TAATACT12650.014.0395264
CGAGCCC16500.013.79090815
GCCCACG17050.013.67155518
Produced by FastQC (version 0.11.5)