FastQCFastQC Report
Fri 29 Apr 2016
ERR1042739.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042739.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3732134
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT153000.4099531260131603No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT142200.38101525829458427No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT91320.24468574815373723No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83250.22306273033069016No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAACGG8450.016.63905335
TAACGGC8650.016.25433536
GGGTAAG5050.015.3861391
GTATTAG15450.015.0873781
GGGGTTA9800.014.9132656
TAGGGGT8900.014.7584284
ATTAGAG11600.014.5129323
GGGTTAG10250.014.2585367
ATAGGGG9400.014.1702143
AACGGCC9950.014.13065337
CGTATTA1501.3076351E-613.56666715
TTTAACG10400.013.51923134
TATTAGA13000.013.5192312
GTAAGAT5800.013.3965533
ATGCTAG10500.013.39047715
TAATACT14700.013.3401374
CTAGTAC1254.8500835E-513.3200013
TTATGCG5500.013.1181824
CCTCTAG10950.013.0091321
TCTAATA12450.012.9277112
Produced by FastQC (version 0.11.5)