FastQCFastQC Report
Fri 29 Apr 2016
ERR1042736.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042736.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11351020
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT504550.44449749890318224No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT454840.4007040776952203No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT338470.29818465653306925No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT211470.1863004381985055No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTG206870.18224793895174177No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAG140530.12380385198863186No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCG132800.11699389129787455No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGT124740.10989320783506681No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG42650.014.9214541
TTAACGG28050.014.707664535
TAACGGC28550.014.45008736
TATTAGA36650.014.08322052
GGGGTTA22700.013.9361236
ATTAGAG34700.013.5417863
TAGGGGT24150.013.2525874
ATAGGGG23850.013.18658353
TTTAACG32800.013.08536534
GGGTTAG25000.012.8767
AACGGCC33000.012.78181837
TCTAATA35550.012.7496482
AATACTG41500.012.6602415
CTCTAAT34500.012.4942031
GTTTAGG26250.012.3333341
TGCGTCA20250.012.33333410
TAGGACC23300.012.3068674
TACCCCG18700.012.2673815
TAATACT41800.012.2153114
TACTGGT38900.012.127257
Produced by FastQC (version 0.11.5)