FastQCFastQC Report
Fri 29 Apr 2016
ERR1042735.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042735.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12428376
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT301660.2427187590719817No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT249980.2011364960313399No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT192290.15471852476944695No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAACGG25500.015.162744535
GGTATCA162250.014.1842841
TAACGGC27150.014.17311336
GTATTAG41700.013.8417271
TTTAACG29250.013.59829134
ATTAGAG33250.013.1864663
TATTAGA36350.013.079782
TAATACT41050.012.438494
GGGGTTA20150.012.2109176
CTCTAAT32750.011.9755721
GTTTAAC33550.011.80029833
TCTAATA35500.011.7774642
GGTAAAC30250.011.74214935
AACGGCC33750.011.5111137
TAGGGGT21700.011.5092164
CTAATAC40650.011.4686353
TTAGAGG48550.011.4315154
AATACTG42050.011.3507735
ATAGGGG21700.011.338713
ATGCTAG31200.011.26602615
Produced by FastQC (version 0.11.5)