FastQCFastQC Report
Fri 29 Apr 2016
ERR1042734.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042734.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13074498
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT512540.39201505097939515No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT444800.3402042663511823No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT325710.24911855124380303No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT204650.1565260861258306No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTG155160.1186737724079349No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAACGG21050.014.23752935
TAACGGC22450.013.43207236
TAATACT39300.013.0394394
CTAATAC38400.012.2851553
TAGGACC22650.012.0883014
TTTAACG26200.011.86259634
AATACTG41150.011.8238165
GTATTAG38600.011.69431
TTAGGAC20100.011.68905453
GGGGTTA23150.011.6673876
ATTAGAG29550.011.4568533
TCTAATA35300.011.4249292
GTTTAGG27100.011.400371
CTCTAAT32650.011.3889751
ATGCTAG29550.011.33164115
ATCTCGT12100.011.3140537
TAGGGGT22800.011.2785094
TATTAGA34750.011.2330932
GTATAGA14500.011.11
GGGTTAG23600.011.0529667
Produced by FastQC (version 0.11.5)