FastQCFastQC Report
Fri 29 Apr 2016
ERR1042733.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042733.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13579225
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT407600.30016440555333607No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT339990.2503751134545602No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT265790.19573281980378113No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG185670.13673092536577014No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC178060.13112677638083176No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT158420.11666350620156893No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG147260.10844506958239516No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAACGG44000.015.55681735
TAACGGC44550.015.28170636
ATTAGAG49250.015.0629433
CTCTAAT51650.014.90029051
TATTAGA54800.014.651462
GTATTAG62250.014.5919691
TTTAACG49400.014.08097334
GGTATCA226200.013.8545531
CTAATAC65750.013.8152083
TCTAATA59700.013.7897822
AACGGCC49900.013.75450937
AATACTG63800.013.686525
TACTGGT59900.013.5893147
ATGCTAG55250.013.56108715
GGTAAAC54050.013.51988935
TAATACT67200.013.3519344
TAGGACC31300.013.1805114
GGGGTTA30950.013.1502436
GTGACAC54800.013.0985424
CACATGT54900.013.04098328
Produced by FastQC (version 0.11.5)