FastQCFastQC Report
Fri 29 Apr 2016
ERR1042732.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042732.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14996279
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT589480.3930841777483601No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT516510.3444254404709328No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT383430.25568342653534254No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT251770.16788831416113292No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG195640.13045902920317765No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC188760.1258712244550798No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG162840.10858693679945539No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAACGG48200.015.50622435
CTACGCT10150.015.31034530
ATTAGAG54400.014.9632353
TAACGGC50200.014.96215236
TACGCTA10400.014.94230731
GTATTAG69800.014.4713461
TATTAGA60800.014.2401312
ACGCTAT11100.014.16666732
CTCTAAT52200.014.1053651
TTTAACG54450.013.96418734
GGTAAAC51600.013.73158935
CGCGATA2457.2759576E-1213.59183814
CTAATAC65250.013.439083
TCTAATA58600.013.3540962
AACGGCC56600.013.30300337
AATACTG64250.013.2739315
TAATACT70450.013.1823994
GGGGTTA35850.013.0557886
CACATGT60750.013.00329228
GTGACAC59600.012.97483224
Produced by FastQC (version 0.11.5)