FastQCFastQC Report
Fri 29 Apr 2016
ERR1042730.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042730.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13020739
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT623600.4789282697395286No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT510890.3923663626158239No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT405440.31138017588709826No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT238610.18325380763718557No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG150620.11567699805671552No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC135860.10434123593138608No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAACGG34500.015.44347835
TAACGGC35250.015.11489436
GGTAAAC40650.014.38130435
ATTAGAG42150.014.3084233
TATTAGA45800.014.2991262
GTATTAG54200.014.2675281
TAATACT53450.014.2254454
ATGCTAG41950.014.15613715
TTTAACG40100.013.93266834
TCTAATA48500.013.4649492
CTCTAAT45350.013.4619631
AATACTG51950.013.4254095
CTAATAC54950.013.332123
GGGGTTA29550.013.2724196
AACGGCC41150.013.1725437
TACTGGT48500.013.0835067
ATACTGG52350.013.0401156
GATGCTA46150.012.94799614
GTGACAC43550.012.91389224
GTTTAAC45450.012.6996733
Produced by FastQC (version 0.11.5)