FastQCFastQC Report
Fri 29 Apr 2016
ERR1042729.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042729.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11774977
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1639461.392325437238646No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1597241.3564697408750777No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT1091550.9270081801433667No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT806350.6847996390990828No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTG616790.5238141866434218No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAG403460.34264185823887383No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCG390180.33136370457453973No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGT357840.30389868277449716No Hit
CCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTT315030.26754192386108266No Hit
GAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTG275490.23396224043579872No Hit
GGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAG231790.19684964140481973No Hit
CTTCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGT229720.19509167618756285No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT225720.1916946419513176No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT215940.18338889324369806No Hit
TCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTG215000.1825905901981804No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT210530.1787944044391764No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT189470.16090901918534534No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTA185510.15754595529146256No Hit
GCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCACTG174620.14829752958328496No Hit
AAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAA157210.13351193807002765No Hit
ACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGA145890.12389833118145369No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT142900.12135904808986038No Hit
CAGTTGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA141610.1202635045486713No Hit
GTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAG141480.12015310093599335No Hit
GAGTACGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAA140330.11917645359307283No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGT138700.11779216214180292No Hit
ACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATAC138150.1173250699343192No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA136210.11567750832974028No Hit
CAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGC132570.1125862071747571No Hit
GCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTG131280.11149066363356802No Hit
GAGTACGGGAAGCAGTTGTATCAACGCAAAAAAAAAAAAAAAA129650.11010637218229812No Hit
GTACGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAAAA127900.10862016970394081No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGT23850.017.37526337
TATACAC28250.016.5026553
CACGAGA26350.016.0075921
ACGAGAC26600.015.64849622
CCCACGA28500.014.86491319
CGAGACT29100.014.81271523
GACTAGG28100.014.74733126
TTAACGG10050.014.17412935
AGACTAG29200.014.12842425
ATCTCCG29550.014.02368810
GAGACTA29950.013.89816524
CTAGGCA30450.013.79146228
TACACAT34650.013.7215015
CGAGCCC30300.013.67656815
TAGGCAT31000.013.54677329
TCTCCGA30200.013.47682211
GCCCACG30700.013.4381118
ACTAGGC30600.013.42156927
GTACTAG7400.013.251
ATACACA37700.013.2493374
Produced by FastQC (version 0.11.5)