FastQCFastQC Report
Fri 29 Apr 2016
ERR1042728.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042728.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8245692
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT228380.2769688705326369No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT211720.2567643807214725No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT147330.17867511908036343No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC108430.13149896940123398No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG101200.12273075443516444No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97900.11872866461662647No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG92210.11182809156587464No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAACGG27800.015.63848935
ATTAGAG30600.015.4771243
TATTAGA32900.015.0136792
CTCTAAT24450.014.9815951
TAACGGC28700.014.95470436
GGGGTTA21100.014.9052126
GTATTAG37800.014.7314821
TTAGGAC17400.014.459773
TTTAACG30550.014.29132634
ATAGGGG22150.013.864563
TAGGGGT23300.013.8154524
GTGACAC32750.013.72671724
GGGTTAG23000.013.6739127
CACATGT32600.013.61963228
TCTAATA29800.013.5956372
TAATACT33200.013.484944
AACGGCC31750.013.45984237
CTAATAC33550.013.4545453
TCTATAC7600.013.3881583
CCTCTAG28000.013.3464281
Produced by FastQC (version 0.11.5)