FastQCFastQC Report
Fri 29 Apr 2016
ERR1042727.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042727.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5206357
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT241920.4646627190567224No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT240810.4625307100531139No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT168720.32406536854848794No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114060.2190783305870112No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTG85140.16353085276326612No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCTCG6450.018.6434136
CGAGACA7800.017.07692323
TTAACGG7650.016.92810435
TAACGGC7750.016.70967736
ATCTCGT7400.016.037
ACGAGAC9150.015.36612122
CACGAGA9150.014.35519221
TTAGGAC8450.014.230773
GGACCGT3900.014.230776
TATACAC10750.014.1116293
TACACAT12200.014.1024585
CTAATAC14400.014.0034733
CCCACGA9000.013.977777519
GTATTAG15300.013.7843131
TTTAACG9800.013.78061234
GGGGTTA7700.013.6948056
TAATACT14050.013.5622784
TCTAATA13050.013.4674332
GTACTAG2102.046363E-913.2142871
GTTTAGG9250.013.21
Produced by FastQC (version 0.11.5)