FastQCFastQC Report
Fri 29 Apr 2016
ERR1042726.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042726.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1775037
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT167900.9458957756936898No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT93300.5256228461716573No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84210.4744126460462514No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAG64290.36218963323018055No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTG49350.2780223736181274No Hit
GGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAG44460.25047365209851963No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTA41080.23143179550623455No Hit
GAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTG40870.23024872157594461No Hit
AAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAA35750.2014042524184003No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32880.18523557537110494No Hit
ACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGA32420.18264408009523184No Hit
CCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTT27810.15667279048267727No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGT27150.15295455813033756No Hit
TCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTG26650.15013771543917115No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT25640.14444769320301493No Hit
GTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAG24850.13999708175097195No Hit
CAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGC24650.13887034467450537No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCG24410.1375182601827455No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT23220.13081417457776937No Hit
GGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGCAGAGT19520.10996953866313772No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGCGT759.27043E-619.7333344
CGAACTA4800.018.524
TATACAC3600.017.4722213
GCGAACT5500.016.48181723
GCCGAGT2200.015.97727312
AGCGAAC5850.015.49572722
TAAGACT6950.015.1726624
ATATCGC1101.4526748E-515.1363646
TACACAT3600.014.9027785
TAACGCC750.004105575414.84
GGTCTAT750.004105575414.81
TATACTG2006.184564E-1114.85
GTGCGAC750.004105575414.811
AGGTCGC4250.014.835
TATAGAC1252.9607581E-614.7999993
AAGCGAA6300.014.68253921
AAAGCGA6300.014.68253920
GTAAGAT2800.014.5357153
TTATACA3750.014.3066662
ATAACGC1304.446956E-614.2307693
Produced by FastQC (version 0.11.5)