FastQCFastQC Report
Fri 29 Apr 2016
ERR1042725.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042725.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13527981
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT595930.44051658558657053No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT570690.42185896032822634No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT410920.3037556010760216No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT281740.20826463313335522No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG170440.12599071509636212No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC155230.11474735217324743No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAACGG38700.015.39276535
GTATTAG57250.014.8323141
TAACGGC40200.014.81840736
ATTAGAG44850.014.7257523
CTCTAAT44800.014.6183031
TATTAGA49600.014.322582
AATACTG57350.014.322585
TAATACT59200.014.31254
GGGGTTA29750.014.05378156
ATGCTAG45700.013.84463915
CTAATAC58800.013.8435373
TTTAACG46450.013.42195934
TCTAATA53550.013.3697482
GGTAAAC45250.013.369060535
AACGGCC44900.013.14365337
GGGTTAG32300.013.0015477
TACTGGT51850.012.9517847
ATTTCGG29150.012.69296729
GATGCTA50300.012.65208714
ATACTGG56350.012.6397516
Produced by FastQC (version 0.11.5)