FastQCFastQC Report
Fri 29 Apr 2016
ERR1042724.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042724.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16188576
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT328690.20303824128817755No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT281140.17366567633867241No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT217020.13405749832474456No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG199950.123513025481673No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC197510.12200578976186663No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG167960.10375217684371991No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAACGG47900.016.41440635
TAACGGC48650.016.04727636
GTATTAG74450.015.257221
GGGGTTA36600.015.0122966
TAATACT67050.015.0096944
TCTAATA59550.014.818642
CTCTAAT54250.014.7317971
ATTAGAG59550.014.6943743
TATTAGA62750.014.6820722
ATGCTAG54550.014.37946815
TTTAACG56450.014.22320734
AATACTG66050.014.1725975
CTAATAC70200.013.8881773
GTTTAAC59850.013.69339933
AACGGCC57700.013.65857937
TACTGGT63600.013.6132077
GATGCTA58500.013.50341914
GGTATCA218300.013.4322031
GGTAAAC56750.013.39823835
ATAGGGG39200.013.3558663
Produced by FastQC (version 0.11.5)