FastQCFastQC Report
Fri 29 Apr 2016
ERR1042723.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042723.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12303075
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT760480.6181218922911549No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT749160.6089209404965832No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT505400.41079161104032935No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT327790.2664293276274427No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTG322590.2622027419974275No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAG214010.17394838282299344No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGT197280.16035015636334818No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCG195830.159171589216517No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG182400.14825561902207374No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC172900.1405339721980074No Hit
CCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTT154920.1259197395773008No Hit
GAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTG154320.12543205661999135No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG137240.11154934843524891No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG133400.1084281775084684No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTT130640.10618483590484491No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGGC42900.015.86946336
TTAACGG42950.015.76484235
GGGGTTA38150.015.0812596
ATTAGAG49250.014.912693
CTCTAAT49900.014.9038081
GGTAAAC48400.014.83057835
GTATTAG60600.014.74504851
TAGGACC29700.014.7003364
TATTAGA53000.014.6254712
AACGGCC46800.014.58653937
GTGACAC47700.014.54402524
AATACTG56400.014.5310285
TAATACT65850.014.2437354
CACATGT49650.014.23363628
TTTAACG48550.014.21318234
TCTAATA56250.014.2082
CTAATAC62600.014.037543
GGGTTAG42000.013.8757
TAGGGGT40400.013.8749994
TACTGGT54650.013.845387
Produced by FastQC (version 0.11.5)