FastQCFastQC Report
Fri 29 Apr 2016
ERR1042721.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042721.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11220208
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTCTAAGTGGGTGGTAAATTCCATCTAAAGCTAAATATTGGC607820.5417190126956648No Hit
CTGTACTTGTTCGCTATCGGTCTCTCGCCAATATTTAGCTTTA511930.4562571389050898No Hit
TCACTGTACTTGTTCGCTATCGGTCTCTCGCCAATATTTAGCT460320.4102597741503545No Hit
TTCCATCACTGTACTTGTTCGCTATCGGTCTCTCGCCAATATT446120.39760403728700927No Hit
ACTTAGAGCTGCATTCCCAAACAACTCGACTCTTCGAAGGCAC435910.38850438423244915No Hit
GTACTTGTTCGCTATCGGTCTCTCGCCAATATTTAGCTTTAGA431310.3844046384879853No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT345650.30806024273346805No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT302630.26971870753198157No Hit
GTTTGGGAATGCAGCTCTAAGTGGGTGGTAAATTCCATCTAAA246960.22010287153321934No Hit
CTATCGGTCTCTCGCCAATATTTAGCTTTAGATGGAATTTACC236400.21069128130245No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT232410.207135197493665No Hit
GGTAAATTCCATCTAAAGCTAAATATTGGCGAGAGACCGATAG196730.17533543050182313No Hit
CGCCAATATTTAGCTTTAGATGGAATTTACCACCCACTTAGAG195410.17415898172297697No Hit
GTCATAGAGGGTGAGAATCCCGTGTGGCGAGGAGTGCGGTTCT167350.14915053268174708No Hit
GAATTTACCACCCACTTAGAGCTGCATTCCCAAACAACTCGAC154020.13727018251355055No Hit
TTCCCAAACAACTCGACTCTTCGAAGGCACTTTACAAAGAACC134280.11967692577535105No Hit
ATATTTAGCTTTAGATGGAATTTACCACCCACTTAGAGCTGCA128910.11489091824322685No Hit
GTGCGGTTCTTTGTAAAGTGCCTTCGAAGAGTCGAGTTGTTTG125410.11177154648113474No Hit
ATTCCATCTAAAGCTAAATATTGGCGAGAGACCGATAGCGAAC122440.10912453672873086No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT122400.10908888676573554No Hit
CACTGTACTTGTTCGCTATCGGTCTCTCGCCAATATTTAGCTT121700.10846501241331712No Hit
ATGCAGCTCTAAGTGGGTGGTAAATTCCATCTAAAGCTAAATA113230.1009161327490542No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCATCA109450.017.7140242
GGTATCA223100.017.206411
ATCACTG120150.016.6446115
CCATCAC124750.015.8084163
CATCACT131050.015.3449074
TTAACGG20300.014.49014835
TATTAGA27400.013.9087592
CGACTCT139650.013.525599527
GGTACGG12700.013.25590411
GTATTAG36050.013.2399441
TCGACTC147550.013.01457226
CTCGACT147700.012.98882925
TTAGAAC19100.012.9790583
TACGGAC13000.012.9513
CTCTTCG143800.012.8650930
TAGAACT21050.012.8313534
CGAAGGC140250.012.79500935
GACTCTT148150.012.66216628
CGGTCTA12400.012.53225833
TCTTCGA147350.012.49236631
Produced by FastQC (version 0.11.5)