FastQCFastQC Report
Fri 29 Apr 2016
ERR1042720.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042720.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9930729
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT554900.5587706602405523No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT505730.5092576788672815No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT271150.2730413849778803No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT248910.25064625164980336No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTG227540.22912718693662873No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAG171680.17287754000738514No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCG141450.14243667307807917No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGT134200.13513610128722675No Hit
GAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTG120860.12170304919205831No Hit
CCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTT113690.11448303543476013No Hit
GGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAG102220.1029330273739219No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG10650.014.417845
TATACAC17800.014.134833
TTAACGG9100.012.60439535
TATACCG5450.012.2201835
CTACACT18350.012.0980924
GTATTAG21300.011.7253521
TAATACT17900.011.4720664
TAACGGC10000.011.28536
TTATGCG7750.011.2193564
CGCTATA3301.0913936E-1111.2121212
GTACTAG4700.011.0212771
TACACAT24700.010.7854255
ATTAGAG15150.010.7458753
GGGGTTA10150.010.5714286
GTATACT6150.010.5284564
CACGAGA17400.010.52586321
TACACTG25550.010.4990225
CGAACTA9500.010.32105324
TATTAGA17650.010.2719552
TTATACC11600.010.2068974
Produced by FastQC (version 0.11.5)