FastQCFastQC Report
Fri 29 Apr 2016
ERR1042718.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042718.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12395287
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT281720.22727993309069808No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT271270.21884930941897512No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTG185310.14950037058440035No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT170100.1372295776612514No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAG129330.10433804396784035No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCG128480.10365229945865716No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAACGG28000.014.20535735
GGGGTTA21750.014.2045976
TATTAGA36950.013.6184032
TAACGGC29550.013.52284336
GTATTAG47350.013.2449841
ATTAGAG35400.013.01271253
TCTATAC9550.012.9790573
TAGGGGT21600.012.5902784
TTTAACG33200.012.31475934
TAATACT40200.011.9651754
TCTAATA32950.011.9590282
GGGTTAG25700.011.8054487
CTCTAAT32150.011.6811811
GTTTAAC35500.011.67323933
TTAGAGG55800.011.6702514
TGCGTCA20800.011.65144310
ATAGGGG23850.011.635223
TATACTG11350.011.5726875
CTAATAC40950.011.4749693
AATACTG39500.011.4746845
Produced by FastQC (version 0.11.5)