FastQCFastQC Report
Fri 29 Apr 2016
ERR1042717.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042717.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14750271
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT442090.2997165272421097No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT409800.2778254040213905No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT289970.19658621865320303No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT186220.1262485279084025No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG167120.11329961327490186No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC160210.10861495358288673No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAACGG38950.015.15147635
TAACGGC40550.014.50801536
TATTAGA52150.014.0479382
GTATTAG59850.013.9097741
ATTAGAG48350.013.8510863
GGGGTTA31400.013.8455426
TAATACT59900.013.5893144
TAGGGGT31900.013.4545454
ATGCTAG44800.013.29687515
TTTAACG46450.013.10333634
TTAGGAC35000.013.0028573
AACGGCC45600.012.98245637
TCTAATA52900.012.974482
TAGGACC34550.012.850944
GGTAAAC46400.012.7187535
GTTTAGG36700.012.7029971
CTAATAC60650.012.6586983
TACCCCG23850.012.6436065
AATACTG59100.012.6150595
TACTGGT53350.012.5876287
Produced by FastQC (version 0.11.5)