FastQCFastQC Report
Fri 29 Apr 2016
ERR1042716.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042716.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13459413
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT338070.2511773730399684No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT334980.2488815819828101No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTG231540.17202830465191907No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT208940.15523708203322092No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCG164650.12233074354728544No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT162190.1205030263949847No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGT162170.12048816690594158No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAG156450.11623835303961622No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAACGG15250.014.55737735
TAACGGC16800.013.10416636
TATACTG14700.012.3333335
TACTCCG28650.012.1396165
TATTAGA26400.012.1231062
GTATTAG34400.011.9927321
TAATACT27800.011.8453244
TTTAACG19600.011.70408234
TCTAATA20550.011.7031632
CTAGTAC7900.011.4746853
GGGGTTA15000.011.4700016
CTAATAC31200.011.3846163
GGACCGT9150.011.3224046
TAGACTG12100.011.314055
GAACCGT7850.011.3121026
ATTAGAC6100.011.2213123
TTAGGAC15000.011.13
ATTAGAG24050.011.0769233
TTAATAC12950.011.03
AACGGTA3553.6379788E-1210.94366318
Produced by FastQC (version 0.11.5)