FastQCFastQC Report
Fri 29 Apr 2016
ERR1042715.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042715.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18685642
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT441160.23609571455987435No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT375260.20082799402878423No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT286050.1530854545966363No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAACGG40050.014.27340835
CTCTAAT57150.014.0489941
GTATTAG65750.013.9277571
TCTAATA63400.013.7145112
TAATACT71950.013.7046574
GGGGTTA39200.013.5918386
TAACGGC41900.013.46658736
TATTAGA53750.013.3888372
CTAATAC74700.013.3239633
AATACTG70300.013.2105265
GGTAAAC54800.013.19981835
ATTAGAG50650.012.9299123
ATGCTAG57500.012.70869515
TAGGGGT41000.012.3182944
GATGCTA61200.012.1519614
TACTGGT66150.012.1375677
TATACTG25150.012.1371775
GGGTTAG42950.012.0174627
GGTATCA280200.011.97021
TAGGACC40150.011.8879214
Produced by FastQC (version 0.11.5)